Control:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Expressing:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Recombinant:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Derivative Assay:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Knock-In:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Real-time Polymerase Chain Reaction:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Software:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Microscopy:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
Fluorescence:Article Title: AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice.
Article Snippet: .. By integrating GO-ATeam into the ROSA26 locus under the control of a carefully selected ubiquitous promoter, we ensured both consistent expression across generations and REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Kusabira Orange antibody MBL PM051M Mouse anti-GAPDH antibody Proteintech 60004-1-Ig HRP-conjugated anti-mouse IgG secondary antibody Proteintech SA0001-1 HRP-conjugated anti-rabbit IgG secondary antibody Vector Labs PI-1000 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose (2DG) FUJIFILM Wako 046–06483 Antimycin A Sigma-Aldrich A8674 proAX Takahisa Anada (Kyushu University) MTA required Alexa Fluor 647–labeled BSA Jackson ImmunoResearch 009-600-051 Experimental models: Cell lines Mouse embryonic fibroblast (MEF) cells Derived in-house Not specified Experimental models: Organisms/strains AVID mouse This study MTA required GO-ATeam2 sensor Hiromi Imamura (Yamaguchi University) MTA required Oligonucleotides GO-ATeam knock-in allele P1 primer Eurofins genomics 5′-AGAGCCTCTGCTAACCATGTTCATGCCTTC GO-ATeam knock-in allele P2 primer Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA qPCR reactions primer1 (Kusabira Orange) Eurofins genomics 5′-AGAGATGACACTACGCGTCACAA qPCR reactions primer2 (Kusabira Orange) Eurofins genomics 5′-GTGACACTAAGTCAAACGCGAAA TaqMan probe (Kusabira Orange) Eurofins genomics 5′-CCGAGGGCGGGCCAATGC Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software N/A Metamorph Molecular Device N/A Other Two-photon microscope (Leica TCS SP8 MP) Leica Microsystems N/A Leica M165FC Fluorescence Stereo Microscope (macro) Leica Microsystems N/A Deposited metabolomics dataset jPOST/ProteomeXchange JPST003962/PXD066645 Cell Reports 44, 116246, September 23, 2025 9 physiological detection of ATP concentrations. ..
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